Urea Lactodifucotetraose Trimethylamine L-carnitine and Choline Analyzed by LCMS - AppNote
March 19, 2014
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Human Plasma Extract
Urea, Lactodifucotetraose, Trimethylamine, L-carnitine & Choline
Using this Method it is possible to analyze Compounds present in a Human Plasma Samples. The mechanism of retention of these polar compounds on the Column used is unknown at this time.
It is possible to Retain and Separate steroids and other Compounds based on the Shape of the Molecules.
Post Time: 3 minutes
Flow rate: 0.4 mL/minute
Detection: ESI – POS - Agilent 6210 MSD TOF Mass Spectrometer
Injection vol.: 1μL
Sample Preparation: Proprietary Lyophilized Plasma Sample was reconstituted in 60 µL 80% Acetonitrile / 20% DI Water mixture.
Attachment No 302 Plasma Extract Analyzed by LCMS pdf 0.4 Mb Download File
Urea, Lactodifucotetraose, Trimethylamine, L-carnitine & Choline
Using this Method it is possible to analyze Compounds present in a Human Plasma Samples. The mechanism of retention of these polar compounds on the Column used is unknown at this time.
It is possible to Retain and Separate steroids and other Compounds based on the Shape of the Molecules.
Peaks:
1. Urea
61.0396 m/z [M + H]+
2. Lactodifucotetraose
635.2393 m/z [M + H]+
3. Trimethylamine N-oxide
76.0757 m/z [M + H]+
4. L-carnitine
162.1125 m/z [M + H]+
5. Choline
105.1148 m/z [M + H]+
Method Conditions
Column: Cogent UDC Cholesterol™, 4μm, 100Å
Catalog No.: 69069-05P-2
Dimensions: 2.1 x 50mm
Mobile Phase:
—
A: DI Water / 0.1% Formic Acid (v/v)
—
B: Acetonitrile / 0.1% Formic Acid (v/v)
Gradient:
| Time (minutes) | %B |
|---|---|
| 0 | 90 |
| 1 | 90 |
| 7 | 20 |
| 11 | 20 |
| 12 | 90 |
Flow rate: 0.4 mL/minute
Detection: ESI – POS - Agilent 6210 MSD TOF Mass Spectrometer
Injection vol.: 1μL
Sample Preparation: Proprietary Lyophilized Plasma Sample was reconstituted in 60 µL 80% Acetonitrile / 20% DI Water mixture.
Attachment No 302 Plasma Extract Analyzed by LCMS pdf 0.4 Mb Download File