HPLC Column, Silica-C, 4um, 4.6mm ID x 150mm Length, 100A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40000‑15P Silica‑C™ HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, providing distinct selectivity, rapid equilibration, and robust performance for HILIC and normal‑phase separations. Unlike conventional bonded‑silica phases, this unmodified silica hydride surface contains no end‑capping or organic ligands, resulting in stable retention behavior and excellent reproducibility—especially advantageous in moisture‑sensitive normal‑phase workflows.
This column is optimized for analytes requiring non‑aqueous or minimally aqueous mobile phases, where traditional silica columns may suffer from variability due to water adsorption.
Application Focus: Silica‑C™ for HILIC & Normal‑Phase Separations
Silica‑C™ stationary phases are selected for the analysis of compounds that are not soluble in water or require non‑polar solvent systems. The Silica‑C™ phase is particularly effective for:
Lipids
Small, very polar compounds
Peptides and proteins
Oligonucleotides
Other analytes requiring non‑polar solvents or normal‑phase conditions
The silica hydride surface supports reproducible retention, reliable selectivity, and rapid equilibration, outperforming conventional silica in many normal‑phase applications.
Key Advantages of Silica‑C™ TYPE‑C™ Technology
Compared to traditional bonded‑silica columns, Silica‑C™ columns provide:
Faster equilibration between injections
No need to control moisture in mobile‑phase solvents
Robust column lifetime under demanding solvent conditions
Unique selectivity, particularly beneficial in normal‑phase HPLC
These attributes make Silica‑C™ a dependable solution for consistent, high‑resolution analytical separations.
Why Choose the 4.6 mm × 150 mm Format
The 4.6 mm ID × 150 mm length configuration is a popular analytical format when expanded resolving power is required for complex mixtures or closely eluting analytes:
Higher resolution than 50 mm and 100 mm formats
Excellent peak capacity for complex samples
Standard analytical flow rates compatible with most HPLC systems
Ideal for Normal Phase methods requiring fast, reliable analysis
This format is frequently selected for Normal Phase workflows where separation confidence is prioritized over shortest run time.
Particle Size and Performance
The 4 µm particle size delivers reliable efficiency with manageable backpressure, providing consistent peak shape and reproducibility on standard analytical HPLC systems while supporting high‑resolution separations.
System Requirements and Flow Rates
Typical flow range (4.6 mm ID): ~1.0–1.5 mL/min
System capability: Compatible with standard analytical HPLC systems
Note: No specialized low‑flow or UHPLC‑only equipment is required
Flow‑rate optimization should consider mobile‑phase viscosity, column temperature, and system pressure limits.