Catalog Number - 40036-25P

The 40036‑25P Amide HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, delivering excellent selectivity, faster equilibration, and robust column lifetime for demanding HILIC separations. The amide stationary phase provides strong, reproducible interaction with amine‑containing and highly polar analytes, while the silica‑hydride surface minimizes moisture‑layer instability commonly seen with conventional amide‑bonded silica phases.

This column is selected when extended retention or enhanced separation between closely related compounds is required, particularly in workflows relying on standard analytical LC systems.

Application Focus: Amide Chemistry for High‑Resolution HILIC Separations

Amide stationary phases are specifically chosen for highly polar analytes that benefit from stable and reproducible HILIC retention. The Amide phase is especially effective for:

• Sugars and sugar alcohols

• Amine‑containing compounds

• Polar metabolites

• Other HILIC‑appropriate polar analytes

The TYPE‑C™ silica hydride backbone supports consistent retention, improved robustness, and superior reproducibility, particularly under high‑organic mobile‑phase conditions.

Key Advantages of Cogent TYPE‑C™ Amide Technology

Compared to traditional amide HILIC columns, this column provides:

• Faster equilibration between injections and gradients

• Improved retention stability across mobile‑phase compositions

• Robust column lifetime under extended HILIC operation

• Reduced moisture sensitivity, improving run‑to‑run consistency

These attributes make the Amide TYPE‑C™ column well suited for routine analysis and high‑confidence method development.

Why Choose the 4.6 mm × 250 mm Format

The 4.6 mm ID × 250 mm length configuration is selected when maximum resolving power and extended retention are required for complex polar mixtures:

• Highest separation efficiency within the 4.6 mm Amide family

• Extended retention times for closely related compounds

• Standard analytical flow rates compatible with most LC systems

• Excellent compatibility with ELSD, Corona CAD, and UV detection, particularly for nitrogen‑containing compounds

This format is often chosen when separation confidence and peak resolution take priority over shortest possible run time.

Particle Size and Performance

The 4 µm particle size provides a practical balance of efficiency, robustness, and manageable backpressure, enabling reliable high‑resolution separations on standard analytical HPLC systems.

System Requirements and Flow Rates

• Typical flow range (4.6 mm ID): ~1.0–1.5 mL/min

• System capability: Compatible with standard analytical HPLC systems

• Note: No specialized low‑flow or UHPLC‑only equipment is required

Flow rates should be optimized based on mobile‑phase viscosity, column temperature, and system pressure limits.