HPLC Column Set, PEEK with a 7.5 mm ID. Includes, a 100 mm x 7.5 mm black, column body tube (5/8-18 threads), plus 2 PEEK 5 um frits, 2 frit spacers & 2 end fittings. ARE-Applied Research brand. 1 EA.

Additional Info:

The 49333‑100 All‑PEEK HPLC Column Set is designed for professional column packers and preparative chromatographers who require bioinert, high‑capacity column hardware for semi‑preparative and scale‑up separations. The 7.5 mm ID format increases sample loading capacity, while the 100 mm length provides enhanced resolution and separation efficiency for more demanding applications.

PEEK (polyether ether ketone) column hardware provides a fully metal‑free flow path, eliminating interactions with stainless steel that can affect sensitive compounds. This makes it ideal for proteins, peptides, nucleic acids, ions, and metal‑sensitive small molecules, where recovery, accuracy, and reproducibility are critical.

✅ Why Choose All‑PEEK Column Hardware

  • True Bioinert Flow Path Prevents interaction with metal surfaces

  • Higher Sample Loading Capacity (7.5 mm ID) Supports semi‑prep and scale‑up workflows

  • Improved Recovery & Peak Shape Minimizes adsorption and secondary interactions

  • Pack Any Stationary Phase Complete flexibility for custom column packing

  • Reusable & Repackable Design Ideal for process development and optimization


Performance & Design

The column body is manufactured using a proprietary extrusion process that produces a smooth, mirror‑like internal surface, supporting:

  • Consistent and reproducible packing

  • Reduced flow disruption

  • Improved chromatographic efficiency

Constructed from graphite-filled PEEK, the graphite is incorporated before extrusion, not applied as a coating.

Key advantages:

  • No chemical coatings or treatments

  • Uniform internal surface chemistry

  • Durable black exterior for long-term use


Included Components

  • 100 mm × 7.5 mm ID All‑PEEK column body (5/8‑18 threads)

  • 2 × 5 µm PEEK frits

  • 2 × frit spacers

  • 2 × PEEK end fittings


Use Case Positioning

7.5 mm ID / 100 mm Length

  • Higher resolution with increased loading capacity

  • Ideal for semi‑preparative purification and method scale‑up

  • Balanced for throughput and separation efficiency


Typical Applications

  • Semi‑Preparative Protein & Peptide Purification

  • Nucleic Acid Analysis and Isolation

  • Ion Chromatography of metal‑sensitive compounds

  • Scale‑up of analytical methods to preparative workflows

  • Custom stationary phase evaluation

  • Bioinert LC and LC‑MS separations


⚠️ When to Use PEEK Column Hardware

Choose All‑PEEK columns when:

  • Analytes are sensitive to stainless steel interaction

  • Higher sample loading and throughput are required

  • Working with biomolecules or coordination-sensitive analytes


Packing & Compatibility

For optimal performance:

  • Use with ARE‑Applied Research™ All‑PEEK slurry reservoir kits

  • Select frit porosity appropriate for particle size and application scale

  • Maintain a fully bioinert packing environment

Read guidelines for packing All‑PEEK HPLC columns and achieving consistent column performance


Specifications

  • Hardware Type: All‑PEEK Column Set

  • Internal Diameter: 7.5 mm

  • Length: 100 mm

  • Thread Type: 5/8‑18

  • Material: Extruded PEEK (graphite‑filled)

  • Frits: 5 µm PEEK

  • Additional Components: Frit spacers included

  • Flow Path: Metal‑free (bioinert)

  • Surface Treatment: None (no coatings)

  • Reusability: Repackable

  • Brand: ARE‑Applied Research™

  • Unit: 1 Each

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