Catalog Number - 49334-05

The 49334‑05 All‑PEEK HPLC Column Set is designed for professional column packers and LC‑MS practitioners who require complete control over bioinert column construction in a low‑flow analytical format. This 2.1 mm ID hardware set enables in‑house packing of custom HPLC columns using virtually any stationary phase.

Constructed entirely from PEEK (polyether ether ketone), this column provides a fully metal‑free flow path, helping prevent interactions between analytes and stainless steel surfaces. This is critical for proteins, peptides, nucleic acids, ions, and metal‑sensitive small molecules, where adsorption or catalytic effects can compromise recovery and performance.

✅ Why Choose All‑PEEK Column Hardware

• True Bioinert Flow Path Eliminates metal contact to protect sensitive analytes

• Improved Recovery & Peak Integrity Reduces adsorption and secondary interactions

• Pack Any Stationary Phase Enables full flexibility for custom separations

• Reusable & Repackable Design Ideal for method development and LC‑MS workflows

• Optimized for 2.1 mm Low‑Flow Systems (1/4‑28 Threads) Suitable for solvent-efficient and MS-sensitive applications

Performance & Design

The column body is manufactured from extruded PEEK tubing using a proprietary process that creates a smooth, mirror-like internal surface, helping to:

• Improve packing consistency

• Reduce flow disruption

• Enhance chromatographic efficiency

Unlike coated or treated hardware, this column is:

• Not chemically modified

• Not surface coated

• Inherently bioinert based on material composition

A controlled amount of graphite is incorporated into the PEEK prior to extrusion, producing the black finish while maintaining structural integrity and long-term durability.

Included Components

• 1 × 50 mm × 2.1 mm ID PEEK Column Body (1/4‑28 threads)

• 2 × 2 µm PEEK Frits

• 2 × PEEK End Fittings

✔ Complete hardware set for packing one column

Use Case Positioning

2.1 mm ID / 50 mm Length

• Short column format → Fast method development with improved resolution vs 30 mm

• Low-flow design → Ideal for LC‑MS applications

• Reduced solvent usage → Efficient analytical workflows

Typical Applications

• Protein & peptide analysis

• Nucleic acid separations

• Ion chromatography & metal‑sensitive analytes

• LC‑MS method development and validation

• Bioanalytical workflows

• Custom stationary phase evaluation

⚠️ When to Use PEEK Column Hardware

Choose All‑PEEK columns when:

• Samples may interact with or adsorb to metal surfaces

• High recovery and reproducibility are required

• Working with biomolecules or coordination-sensitive analytes

• A fully bioinert LC system is necessary

Packing & Compatibility

For optimal results:

• Use with All‑PEEK Slurry Reservoir Kits designed for 2.1 mm columns

• Select frit porosity appropriate for particle size

• Maintain a fully bioinert packing and flow path environment

Specifications

• Hardware Type: All‑PEEK Column Set

• Internal Diameter: 2.1 mm

• Length: 50 mm

• Thread Type: 1/4‑28

• Material: Extruded PEEK (graphite‑filled)

• Frits: 2 µm PEEK

• Flow Path: Metal‑free (bioinert)

• Surface Treatment: None

• Reusability: Repackable

• Brand: ARE‑Applied Research™

• Unit: 1 Each

Resources & Ordering Options

• View All‑PEEK Slurry Reservoir Kits for 2.1 mm Column Packing

• Read Guidelines for Packing All‑PEEK HPLC Columns and Achieving Consistent Performance