HPLC Column Set, PEEK with a 4.6 mm ID. Includes, a 50 mm x 4.6 mm black, column body tube (1/2-20 threads), plus 2 PEEK 2 um frits & 2 end fittings. ARE-Applied Research brand. 1 EA.

Additional Info:

The 49335‑05 All‑PEEK HPLC Column Set is designed for professional column packers and advanced chromatographers who require complete control over bioinert column construction. This 4.6 mm ID hardware set enables in‑house packing of custom HPLC columns using virtually any stationary phase.

PEEK (polyether ether ketone) column hardware provides a fully metal‑free flow path, helping prevent undesirable interactions between analytes and stainless steel surfaces. This is critical for proteins, peptides, nucleic acids, ions, and metal‑sensitive small molecules, where adsorption or catalytic effects can compromise results.

✅ Why Choose All‑PEEK Column Hardware

  • True Bioinert Flow Path Eliminates metal contact to protect sensitive analytes

  • Improved Recovery & Peak Integrity Reduces adsorption and secondary interactions common with stainless steel columns.

  • Pack Any Stationary Phase Full flexibility for method development and custom separations

  • Reusable & Repackable Design Ideal for R&D, method optimization, and specialized workflows

  • Compatible with Standard Packing Systems Uses 1/2‑20 threaded format for 4.6 mm ID columns


Performance & Design

The column body is manufactured from extruded PEEK tubing using a proprietary process that creates a smooth, mirror‑like inner surface. This highly uniform internal bore helps:

  • Improve packing consistency

  • Reduce flow disruption

  • Enhance chromatographic efficiency

Unlike coated or treated hardware, this column is:

  • Not chemically modified

  • Not surface coated

  • Fully inherent in its bioinert properties

A controlled amount of graphite is incorporated into the PEEK prior to extrusion, producing the durable black finish while maintaining structural integrity and long-term usability.


Included Components

  • 50 mm × 4.6 mm ID All‑PEEK column body (1/2‑20 threads)

  • 2 × 2 µm PEEK frits

  • 2 × PEEK end fittings


Use Case Positioning

4.6 mm ID / 50 mm Length

  • Short bed length → Fast method development

  • Analytical scale → Compatible with standard HPLC systems

  • Ideal for screening and optimization workflows


Typical Applications

  • Protein & Peptide Analysis

  • Nucleic Acid Separations

  • Ion Chromatography & Metal‑Sensitive Compounds

  • Bioanalytical Method Development

  • Custom Stationary Phase Evaluation

  • LC‑MS Compatible Workflows (metal‑free requirement)


⚠️ When to Use PEEK Column Hardware

Choose All‑PEEK columns when:

  • Samples may adsorb to or react with metals

  • Recovery and reproducibility are critical

  • Working with biomolecules or coordination-sensitive analytes


Packing & Compatibility

For optimal results:

  • Use with ARE‑Applied Research™ All‑PEEK Slurry Reservoir Kits

  • Select frit porosity based on particle size

  • Maintain a fully bioinert packing environment


Specifications

  • Hardware Type: All‑PEEK Column Set

  • Internal Diameter: 4.6 mm

  • Length: 50 mm

  • Thread Type: 1/2‑20

  • Material: Extruded PEEK (graphite‑filled)

  • Frits: 2 µm PEEK

  • Flow Path: Metal‑free (bioinert)

  • Surface Treatment: None (no coatings)

  • Reusability: Repackable

  • Brand: ARE‑Applied Research™

  • Unit: 1 Each


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