Catalog Number - 72530-10P

The 72530‑10P Cogent C30™ HPLC Column is designed for shape‑selective separation of long‑chain hydrophobic compounds, delivering improved resolution of positional and geometric isomers compared to conventional C18 phases.

This column utilizes a 30‑carbon bonded stationary phase on high‑purity silica (non‑end capped), providing stronger hydrophobic interaction and a distinct selectivity profile that enables separations not achievable with standard reversed‑phase columns.

With a 200 Å pore size, it is particularly effective for larger molecules, including carotenoids, vitamins, peptides, and some proteins, while maintaining consistent retention and peak shape.

✅ Comparable selectivity and application performance to YMC® C30 columns, making it a reliable alternative for USP L62 methods and equivalent workflows

100 mm Length – Increased Resolution for Analytical Methods

The 100 mm length offers a practical balance between speed and resolving power, providing improved separation over short columns while maintaining efficient run times.

• Better separation than 50 mm screening columns

• Improved resolution of closely related isomers

• Increased peak capacity for complex samples

✅ Use when additional resolution is needed without moving to longer 150 mm columns

Flow Rate & System Requirements (4.6 mm ID, 5 µm)

⚠️ Compatible with standard HPLC systems

• Typical flow rate range: ~0.8–1.5 mL/min

• Lower backpressure compared to 3 µm columns

• Standard analytical detector compatibility

• Stable flow delivery required for consistent results

✅ Sample preparation requirement: filter samples using a 0.2 µm syringe filter prior to injection

Why Choose C30 for Hydrophobic Separations

Shape Selectivity for Isomers

• Resolves positional and geometric isomers

• Provides separation beyond traditional C18 capability

Effective for Larger Molecules

• 200 Å pore improves interaction with complex analytes

• Ideal for carotenoids, vitamins, and peptides

Reversed‑Phase Alternative to Normal Phase

• Avoids instability and reproducibility issues

• Maintains robust, repeatable operation

Cogent C30™ Advantages

Extended C30 Ligand

• Stronger hydrophobic interaction than C18

• Unique selectivity for long‑chain compounds

High Purity Silica

• Consistent column‑to‑column performance

• Reliable chromatographic reproducibility

Method Transfer Capability

• Comparable to YMC® C30 column chemistry and applications

• Suitable for transferring L62 methods with minimal adjustment

Typical Applications

• Carotenoid and vitamin analysis

• Isomer and positional compound separations

• Peptide and large molecule separations

• Hydrophobic compound profiling

• Method development and optimization

Important Usage Notes

• Designed for standard analytical HPLC systems (4.6 mm ID)

• Not end‑capped (intentional for selectivity differences)

• Lower backpressure vs smaller particle sizes

• Ideal for routine analytical and method development workflows