Phenolic Compounds found in Rice Extract Analyzed with LCMS - AppNote
January 26, 2014
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Content of Phenolic Compounds in Rice Determined by LCMS
Click HERE for Column Ordering Information.
When commercial Rice Extracts were analyzed, only 6’-O-Feruloylsucrose at 3.01 minutes (Peak not shown for the clarity of the Chromatogram) was found in this particular Rice Extract Sample. Next, the Sample was spiked with the Standards according to the literature referenced below [1] .
Column: Cogent Phenyl Hydride™, 4μm, 100Å
Catalog No.: 69020-05P-2
Dimensions: 2.1 x 50mm
Mobile Phase:
-- A: DI Water / 0.1% Formic Acid (v/v)
-- B: Acetonitrile / 0.1% Formic Acid (v/v)
Gradient:
Post Time: 3 minutes
Injection vol.: 1μL
Flow rate: 0.4mL / minute
Detection: ESI – NEG - Perkin Elmer, Flexar SQ 300 Mass Spectrometer
Sample Preparation: Commercial Rice Extract was spiked with standards at a concentration of 12.5 ppm and was analyzed.
Note: Rice is a staple food in many countries. It contains phenolic compounds which have anticancer, antioxidant, and anti-mutagenic effects. It is important to analyze Rice Extracts to confirm the content of the phenolic compounds in rice.
[1] J.E. Hayes, P. Allen, N. Brunton, M.N. O’Grady, and J.P. Kerry, Food Chemistry, 126, (2011) 948–955.
Attachment No 287 Rice Extracts Analyzed with LCMS pdf 0.3 Mb Download File
Click HERE for Column Ordering Information.
When commercial Rice Extracts were analyzed, only 6’-O-Feruloylsucrose at 3.01 minutes (Peak not shown for the clarity of the Chromatogram) was found in this particular Rice Extract Sample. Next, the Sample was spiked with the Standards according to the literature referenced below [1] .
This Method is an excellent choice to use for this analysis of these Phenolic Compounds.
Peaks:
1. Gallic Acid
169 m/z [M-H]–
2. Caffeic Acid
179 m/z [M-H]–
3. p-Coumaric Acid
163 m/z [M-H]–
4. Ferulic Acid
193 m/z [M-H]–
5. 3,5-Dimethoxy-4-Hydroxycinnamic Acid
223 m/z [M-H]–
Column: Cogent Phenyl Hydride™, 4μm, 100Å
Catalog No.: 69020-05P-2
Dimensions: 2.1 x 50mm
Mobile Phase:
-- A: DI Water / 0.1% Formic Acid (v/v)
-- B: Acetonitrile / 0.1% Formic Acid (v/v)
Gradient:
| Time (minutes) | %B |
|---|---|
| 0 | 10 |
| 5 | 20 |
| 6 | 20 |
| 7 | 10 |
Injection vol.: 1μL
Flow rate: 0.4mL / minute
Detection: ESI – NEG - Perkin Elmer, Flexar SQ 300 Mass Spectrometer
Sample Preparation: Commercial Rice Extract was spiked with standards at a concentration of 12.5 ppm and was analyzed.
Note: Rice is a staple food in many countries. It contains phenolic compounds which have anticancer, antioxidant, and anti-mutagenic effects. It is important to analyze Rice Extracts to confirm the content of the phenolic compounds in rice.
[1] J.E. Hayes, P. Allen, N. Brunton, M.N. O’Grady, and J.P. Kerry, Food Chemistry, 126, (2011) 948–955.
Attachment No 287 Rice Extracts Analyzed with LCMS pdf 0.3 Mb Download File