Creatine and Creatinine Analyzed with ELSD and UV - AppNote
Date:
13-OCTOBER-2025
Last Updated: 13-OCTOBER-2025
Creatine and creatinine are key biomarkers in clinical diagnostics. Their accurate separation is essential for reliable analysis. This application note describes an HPLC method using ELSD and UV detection optimized for the efficient separation and quantification of creatine and creatinine.
Catalog No.: 70000-7.5P
Dimensions: 4.6 x 75 mm
Mobile Phase: 70% Acetonitrile / 30% DI Water
Flow Rate: 1.0 mL / minute
Detection:
Sample Preparation: Creatinine (0.45 mg/mL) and Creatine (0.26 mg/mL) reference standards in diluent of 50% Acetonitrile / 50% DI Water
Creatine and creatinine are key biomarkers in clinical diagnostics. Their accurate separation is essential for reliable analysis. This application note describes an HPLC method using ELSD and UV detection optimized for the efficient separation and quantification of creatine and creatinine.
Peaks:
1. Creatinine
2. Creatine
1. Creatinine
2. Creatine
Method Conditions:
Column: Cogent Diamond Hydride™, 4 μm, 100 ÅCatalog No.: 70000-7.5P
Dimensions: 4.6 x 75 mm
Mobile Phase: 70% Acetonitrile / 30% DI Water
Flow Rate: 1.0 mL / minute
Detection:
A: UV 210 nm
B: ELSD (Gain 8, Temp 50°C, Nitrogen: 3.5 lb)
Injection Volume: 2 uLB: ELSD (Gain 8, Temp 50°C, Nitrogen: 3.5 lb)
Sample Preparation: Creatinine (0.45 mg/mL) and Creatine (0.26 mg/mL) reference standards in diluent of 50% Acetonitrile / 50% DI Water
Note: Creatine is stored in muscles and used for quick energy during intense activity, while creatinine is a waste product formed when creatine breaks down—making it a key marker for kidney function in medical tests.