HPLC Column, Bidentate C18, 4um, 4.6mm ID x 150mm Length, 100A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40018‑15P Cogent Bidentate C18™ HPLC Column is a long‑format analytical column designed to deliver maximum resolving power while retaining the unique advantages of TYPE‑C™ silica hydride technology. This column is frequently selected when challenging mixtures require greater peak capacity than shorter formats can provide, or when method robustness under aggressive conditions is essential.
The stationary phase consists of an aliphatic C18 chain bonded via two direct silicon–carbon bonds (bidentate attachment) to a silica hydride surface. This non‑traditional C18 chemistry produces a phase that is extremely hydrophobic yet versatile, capable of retaining compounds that often exhibit little or no retention on conventional silica‑based C18 columns.
Unlike standard C18 phases, the Bidentate C18™ can be operated with 100 % aqueous mobile phases without phase collapse and can tolerate very low pH conditions (below pH 2) with no damage to the column. These properties enable method development across a wider operating window than traditional reversed‑phase materials.
Reversed‑Phase and HILIC‑Mode Flexibility
One of the distinguishing attributes of TYPE‑C™ silica hydride columns is their ability to function beyond conventional reversed‑phase chromatography. The 40018‑15P is commonly chosen for:
Reversed‑phase separations requiring enhanced resolution
HILIC‑phase–style methods for small, highly polar compounds, particularly when using UV detection
In HILIC‑like conditions, the hydride surface chemistry enables rapid separations of polar analytes with improved reproducibility compared to traditional silica‑based HILIC phases.
Key Features
Cogent TYPE‑C™ Silica Hydride Technology
Reduced silanol activity and secondary interactions
Faster equilibration than conventional C18 columns
Extremely robust column lifetime
Bidentate C18™ Bonding
Two‑point attachment via direct Si–C bonds
Unique selectivity not achievable with standard C18 phases
Retains both hydrophobic and select hydrophilic analytes
Long‑Format Analytical Performance
4.6 mm ID × 150 mm length
Increased plate count and peak capacity
Ideal for complex separations and closely eluting compounds
Exceptional Method Flexibility
Compatible with 100 % aqueous mobile phases
Stable at very low pH (below 2)
Supports both RP and HILIC‑mode workflows
Typical Applications
High‑resolution analytical HPLC
Complex mixtures requiring maximum separation power
Small, highly polar compounds (HILIC‑mode use)
Low‑pH reversed‑phase methods
UV‑based detection workflows
Method development and optimization