Prep HPLC Column, Amide, 4um, 100A. 30mm ID x 150mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40036‑P30‑150 Amide Preparative HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, providing excellent selectivity, faster equilibration, and robust column lifetime for high‑resolution, large‑scale preparative HILIC separations. The amide stationary phase delivers strong, reproducible interaction with amine‑containing and highly polar analytes, while the silica‑hydride surface minimizes moisture‑layer instability that can compromise retention and reproducibility in conventional amide silica phases.
This column is designed for preparative workflows where greater resolving power than 30 × 50 mm and 30 × 100 mm formats is required, while maintaining high loading capacity and reproducible recovery.
Preparative Use Case: Amide Chemistry for High‑Resolution HILIC Purification
Amide stationary phases are especially effective for preparative purification (“prep”) of highly polar compounds requiring stable HILIC retention at elevated sample loads. This column is commonly selected for purification of:
Sugars and sugar alcohols
Amine‑containing analytes
Polar metabolites
Other HILIC‑appropriate polar compounds requiring fraction collection
The TYPE‑C™ amide phase supports consistent retention behavior and rapid re‑equilibration, improving purity, yield, and repeatability in extended preparative purification runs.
Why Choose the 30 mm × 150 mm Preparative Format
The 30 mm ID × 150 mm length configuration is selected when additional separation confidence and peak capacity are required compared to shorter large‑bore preparative columns:
Higher resolving power than 30 × 50 mm and 30 × 100 mm formats
Improved separation of closely eluting polar analytes
Very high sample loading capacity with large fraction volumes
Ideal when purity requirements exceed what mid‑length prep columns can deliver, but maximum 250 mm resolution is not yet required
This format offers a strong balance between resolution, throughput, and run time for demanding preparative HILIC workflows.
Key Advantages of Cogent TYPE‑C™ Amide Technology
Compared to conventional amide silica phases, this column provides:
Faster equilibration between preparative runs
Improved retention stability under high‑organic HILIC conditions
Robust column lifetime during repeated large‑scale purification
Reduced moisture sensitivity, improving batch‑to‑batch reproducibility
These advantages are particularly valuable in preparative environments where consistency, yield, and separation confidence are critical.
Particle Size and Performance
The 4 µm particle size provides a practical balance between efficiency and permeability, enabling high‑resolution separations with manageable backpressure on large‑scale preparative systems while maintaining strong recovery during fraction collection.
System Requirements
Typical operating flow range (30 mm ID): ~20–60 mL/min (method dependent)
Requires a large‑scale preparative HPLC system capable of:
Sustained high‑flow delivery
Large injection volumes
Dedicated fraction‑collection hardware
Analytical and semi‑preparative HPLC systems are not suitable for 30 mm ID preparative columns.
Made‑to‑Order Columns & Pricing Information
This is a custom, made‑to‑order column.
Columns are manufactured specifically to order
Orders cannot be canceled once placed
Custom columns are non‑returnable