HPLC Column, Diol 2.o, 2.2um, 2.1mm ID x 150mm Length, 120A. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40260‑15P‑2 Diol 2.o™ HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, combining the well‑established selectivity of Diol chemistry with the performance and equilibration advantages of a silica hydride surface. The 2.2 µm particle size delivers increased efficiency, excellent selectivity, and faster equilibration than conventional bonded‑silica diol columns, while typically remaining compatible with standard HPLC systems under normal operating pressures.
This column is classified as “near‑UHPLC,” enabling improved resolution and sharper peak shapes without requiring UHPLC‑rated instrumentation.
Application Focus: Diol Chemistry for HILIC & Polar Compounds
Diol stationary phases are a trusted choice for HILIC and normal‑phase chromatography, particularly for polar analytes that are poorly retained by reversed‑phase methods. The Diol 2.o™ phase is especially suited for:
Amino acids
Polar metabolites
Small biological and biomarker compounds
Other highly water‑soluble or charged analytes
When operated in HILIC mode, this column is considered mass‑spectrometry friendly, allowing the use of MS‑compatible mobile phases while maintaining consistent retention and selectivity for polar compounds.
Cogent TYPE‑C™ Silica Hydride Advantages
Compared to traditional bonded‑silica diol columns, the Diol 2.o™ stationary phase offers measurable operational benefits:
Faster equilibration than conventional silica‑based diol phases
Improved efficiency and peak shape from 2.2 µm particles
Stable and reproducible selectivity across a wide range of mobile‑phase conditions
Reduced water‑layer instability, improving performance during gradient HILIC methods
The silica hydride surface supports more consistent chromatography, particularly in high‑throughput and LC‑MS workflows.
Why Choose the 2.1 mm × 150 mm Format
The 150 mm column length provides the highest resolving power of the Diol 2.o™ 2.1 mm ID formats, making it ideal for complex mixtures where maximum separation confidence is required. This geometry is optimized for:
Maximum resolution of closely eluting polar analytes
Higher peak capacity for complex or multi‑component samples
Excellent reproducibility in demanding HILIC methods
LC‑MS compatibility with reduced solvent flow to the MS source
This format is well suited for metabolomics, detailed biomarker studies, and method development where resolution is prioritized over run time.
Particle Size and Separation Efficiency
The 2.2 µm particle size delivers near‑UHPLC efficiency, producing narrow, symmetrical peaks and enhanced resolving power, while typically remaining within the pressure capabilities of many conventional HPLC systems under common operating conditions.
LC‑MS‑Focused Performance
The narrow internal diameter and stable Diol retention mechanism support:
Improved signal‑to‑noise ratios
Consistent peak symmetry across extended gradients
Reliable quantitative performance
These attributes make the 40260‑15P‑2 column a strong choice for LC‑MS and LC‑MS/MS analysis of polar and semi‑polar compounds requiring high resolution.
System Requirements and Flow Rates
Typical flow range (2.1 mm ID): ~0.2–0.3 mL/min
System capability: Requires an HPLC system capable of accurate, stable low‑flow delivery
Important note: Not all standard HPLC pumps or flow cells are suitable for precise operation at reduced flow rates
Final flow‑rate selection should consider mobile‑phase composition, viscosity, column temperature, and system pressure limits.