Catalog Number - 68324-05P-2

Cogent RP Phenyl™ is a reversed-phase HPLC stationary phase designed to provide unique selectivity through π-π interactions between aromatic analytes and the bonded phenyl functionality. Manufactured using a traditional three-carbon linker with a terminal phenyl ring bonded to ultra-high purity silica and fully double end capped, this stationary phase provides chromatographic behavior distinct from conventional alkyl phases. As a USP L11 equivalent phase, it is widely used for method replacement, method development, and applications involving aromatic and structurally related compounds.

68324-05P-2 is an HPLC column packed with 3 µm RP Phenyl™ particles featuring a 100 Å pore size in a 2.1 mm ID x 50 mm format. This popular narrow-bore configuration is optimized for rapid separations, reduced solvent consumption, and excellent compatibility with LC-MS instrumentation. The 3 µm particles provide improved efficiency and sharper peaks than comparable 5 µm columns, while the short 50 mm column length minimizes run times for high-throughput workflows. Typical operating flow rates are approximately 0.2–0.3 mL/min. Because of the low flow rates required by 2.1 mm ID columns, standard HPLC systems must be capable of precise and reproducible low-flow operation.

This column is particularly useful for the separation of positional isomers, tocopherols, flavonoids, polynuclear aromatic hydrocarbons, nitro-aromatic compounds, active pharmaceutical ingredients (APIs), natural products, and related compounds. The unique selectivity of the phenyl phase makes it an excellent choice when conventional C18 or C8 phases do not provide adequate resolution. This column format is especially popular for LC-MS methods where fast analyses, reduced solvent consumption, and enhanced selectivity are desired.

Key Benefits

• RP Phenyl™ stationary phase

• USP L11 equivalent chemistry

• Fully double end capped

• Phenyl bonded phase

• Ultra-high purity silica support

• Enhanced aromatic selectivity

• Excellent isomer separation capability

• 3 µm particle size

• Greater efficiency than 5 µm columns

• Excellent for LC-MS applications

Typical Applications

• USP L11 methods

• LC-MS methods

• Positional isomer separations

• Tocopherol analysis

• Flavonoid analysis

• Polynuclear aromatic hydrocarbon analysis

• Nitro-aromatic compound analysis

• Active pharmaceutical ingredient (API) analysis

• Natural product analysis

• Method development

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP Phenyl™

• USP Classification: L11

• Chromatography Mode: Reversed Phase

• Bonded Phase: Phenyl

• Endcapping: Fully Double End Capped

• Particle Size: 3 µm

• Pore Size: 100 Å

• Column Dimensions: 2.1 mm ID x 50 mm

• Length: 50 mm

• Typical Flow Rate: 0.2–0.3 mL/min

• Brand: Cogent

Important Notes

• Suitable for conventional HPLC and LC-MS systems

• 3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns

• Generates higher backpressure than larger particle columns

• The 2.1 mm ID format is commonly used for LC-MS applications

• Standard HPLC systems must be capable of precise and reproducible operation at approximately 0.2–0.3 mL/min

• Detector flow cells should be optimized for low-volume, narrow-bore chromatography

• The 50 mm length is ideal for rapid analyses and high-throughput workflows

• Provides unique selectivity compared to C8 and C18 phases

• Particularly useful for aromatic compounds and positional isomers

• Commonly used as an alternative for USP L11 methods