Every Day Columns for Normal Phase Chromatography
View Cogent NP HPLC Column Ordering Information
Cogent NP HPLC Columns are high-purity silica columns designed for normal phase chromatography, providing reproducible separations for compounds that may be difficult to retain or resolve using reversed phase methods. Available in multiple stationary phases, particle sizes, pore sizes, and column dimensions, Cogent NP columns are suitable for analytical, semi-preparative, preparative, and purification applications.
Used in both USP methods and laboratory-developed methods, Cogent NP columns are an excellent choice for separating structurally similar compounds, isomers, non-polar compounds, and analytes that require normal phase selectivity.
What is Normal Phase Chromatography?
Normal phase chromatography uses a polar stationary phase and relatively non-polar mobile phases to separate compounds based on differences in polarity and adsorption.
This separation mode is commonly used for:
- Isomer separations
- Lipid analysis
- Natural products
- Chiral method development
- Purification workflows
- Hydrophobic compounds
- Compounds with limited retention in reversed phase chromatography
Available Cogent NP Stationary Phases
- Silica: Unmodified high-purity silica suitable for traditional normal phase applications. Also suitable for HILIC applications when appropriate aqueous-organic mobile phases are used.
- Amino: Polar amino-bonded phase commonly used for carbohydrate, sugar, and polar compound separations.
- Cyano: Versatile cyano phase offering selectivity that can differ significantly from silica and amino phases.
- DIOL: Diol-bonded stationary phase providing unique polar interaction mechanisms and useful alternative selectivity for challenging separations.
Why Choose Cogent NP Columns?
High-Purity Silica
Manufactured using premium spherical silica with low metal content to support reproducible chromatographic performance.
Excellent Peak Shape and Reproducibility
Designed to provide reliable retention, efficient separations, and consistent results from column to column.
Multiple Pore Sizes Available
- 100Å phases for routine analytical applications
- 300Å phases for larger molecules and specialized separations
Easy Method Scale-Up
Methods developed on analytical columns can be transferred to larger dimensions for increased sample loading and purification workflows.
Available Column Formats
- Guard Columns
- Analytical Columns
- Semi-Preparative Columns
- Preparative Columns
- Purification Columns
Common internal diameters include:
- 2.1 mm ID
- 4.6 mm ID
- 10 mm ID
- 21.2 mm ID
- 30 mm ID
- 50 mm ID
Typical Flow Rate Guidelines
Analytical Columns
- 2.1 mm ID: approximately 0.2-0.3 mL/min (system must deliver reliable low-flow performance)
- 3.0 mm ID: approximately 0.4-0.6 mL/min
- 4.6 mm ID: approximately 1.0-1.5 mL/min
Semi-Preparative and Preparative Columns
- 10 mm ID: approximately 4-8 mL/min
- 21.2 mm ID: approximately 15-25 mL/min
- 30 mm ID: approximately 30-50 mL/min
Purification Columns
- 50 mm ID: flow rates dependent on system capabilities and application requirements, often exceeding 80 mL/min
Applications
Cogent NP columns are commonly used for:
- USP methods
- Normal phase chromatography
- Purification methods
- Method development
- Impurity profiling
- Natural product analysis
- Lipid analysis
- Isomer separations
- Carbohydrate analysis
More Information about Cogent NP™ Reversed Phase Columns:
| Download Literature - Ordering Brochure (PDF) | FAQ - General HPLC Tips (Knowledge Base) |
| Ordering Information - Cogent NP™ Columns | Specifications Page |
| USP© Classification List | |
Available in Guard, Analytical & Preparative Sizes.
