Catalog Number - 40231-15P-2

40231-15P-2 Cogent UDA™ (weak cation exchange, WCX) HPLC Column is a highly specialized solution for difficult separations, built on Cogent TYPE‑C™ silica (silica hydride platform). This chemistry delivers unique selectivity not achievable with conventional C18, phenyl, or traditional ion exchange columns.

The UDA™ ligand (Undecanoic Acid) features an 11‑carbon hydrophobic chain with a terminal carboxylic acid group, enabling true multi‑mode retention:

• Reversed phase (hydrophobic interactions)

• Weak cation exchange (ionizable acid group)

• HILIC (polar interactions)

This combination provides powerful selectivity for polar, ionizable, and basic compounds, particularly in complex matrices where co‑elution is common.

The TYPE‑C silica base offers critical advantages over traditional silica:

• Faster equilibration

• Reduced sensitivity to mobile phase moisture

• Improved chemical robustness and stability

150 mm Length – Maximum Resolution for LC‑MS and Complex Separations

The 150 mm length provides enhanced resolving power for the most challenging analytical separations.

• Higher resolution than 50 mm and 100 mm formats

• Improved separation of closely related or co‑eluting compounds

• Increased peak capacity for complex sample matrices

✅ Recommended for LC‑MS workflows requiring maximum resolution and difficult separations

Flow Rate & System Requirements (2.1 mm ID, 2.2 µm)

⚠️ Requires UHPLC or low‑flow capable HPLC system

• Typical flow rate range: ~0.2 – 0.4 mL/min

• Higher backpressure due to 2.2 µm particle size

• Requires precise low‑flow solvent delivery

• Optimized for low‑dispersion systems and small detector volumes

✅ Important: Standard HPLC systems must be capable of stable, accurate low‑flow operation

✅ System Fit: Ideal for UHPLC and LC‑MS platforms with low delay volume

Unique Selectivity – Why Choose UDA™ (WCX)

True Mixed‑Mode Retention

• Hydrophobic (RP)

• Ionic (weak cation exchange)

• Polar (HILIC)

Powerful for Complex Matrices

• Resolves structurally similar compounds

• Separates ionizable and basic analytes effectively

pH‑Controlled Selectivity

• WCX mechanism active at pH ≥ 6

• Retention tunable via pH and ionic strength

✅ Ideal when traditional reversed phase or ion exchange methods are insufficient

TYPE‑C Silica Advantages

Faster Equilibration

• Reduces method cycle time

No Strict Moisture Control Required

• More robust than traditional HILIC phases

Robust Column Lifetimes

• Stable chemistry for longer use

Low Salt Requirements (HILIC Mode)

• Improved MS compatibility

• Cleaner baselines and reduced contamination

Cogent UDA™ Column Advantages

Multi‑Mode Flexibility

• Combines RP, HILIC, and WCX in one column

Near UHPLC Performance (2.2 µm)

• High efficiency with controlled pressure

Optimized for LC‑MS

• Lower salt requirements vs traditional ion exchange

Typical Applications

• LC‑MS method development

• Complex matrix analysis

• Polar and ionizable compound separations

• Basic compound retention and resolution

• Pharmaceutical and bioanalytical workflows

Important Usage Notes

• Packed and shipped in reversed‑phase solvents

• Requires UHPLC or low‑flow capable HPLC system

• Optimized for low‑volume, high‑efficiency systems

• pH control is critical for WCX functionality