Catalog Number - 59051-25P

MicroBee Cyano™ is a traditional cyanopropyl HPLC stationary phase developed for laboratories requiring an economical alternative to legacy cyano columns used in established analytical methods. As part of the Cogent™ Similars product line, it has been widely used for many years when cyano selectivity is preferred over conventional C18 phases. The bonded cyano functionality provides moderate polarity and unique retention characteristics that can improve selectivity for compounds that are difficult to separate using traditional reversed-phase columns.

59051-25P is a 250 mm x 4.6 mm ID analytical column packed with 10 µm, 125Å MicroBee Cyano particles. The 250 mm length provides greater resolving power and higher theoretical plate counts than shorter columns, making it an excellent choice for complex sample matrices, impurity profiling, and methods that require maximum chromatographic resolution. The industry-standard 4.6 mm ID format typically performs optimally at flow rates of approximately 1.0–2.0 mL/min on conventional HPLC systems.

This USP L10 column is manufactured using a non-endcapped cyanopropyl bonded phase attached to Type-A silica. It serves as an excellent alternative for methods developed on Microbondapak™ Cyano and similar cyano columns. The phase is commonly used for polar and moderately hydrophobic compounds and provides selectivity distinct from traditional C18 stationary phases, making it a valuable tool for method development, validation, and difficult separations where alternative retention mechanisms are beneficial.

Key Benefits

• Traditional cyanopropyl stationary phase

• Suitable alternative for USP L10 methods

• Increased resolution from 250 mm column length

• Unique selectivity compared to C18 columns

• Non-endcapped cyano bonded phase

• 125Å pore size for broad analyte compatibility

• Cost-effective Cogent Similars column

• Compatible with legacy cyano methods

Typical Applications

• USP L10 methods

• Reversed-phase HPLC

• Method development

• Pharmaceutical analysis

• Quality control testing

• Impurity profiling

• Polar compound separations

• Legacy cyano column replacement

Specifications

• Product Type: HPLC Column

• Stationary Phase: MicroBee Cyano

• USP Classification: L10

• Particle Size: 10 µm

• Particle Type: Type-A Silica

• Bonded Phase: Cyanopropyl

• Endcapped: No

• Pore Size: 125Å

• Column Dimensions: 4.6 mm ID x 250 mm

• Length: 250 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Brand: Cogent

Important Notes

• USP L10 column classification

• Non-endcapped cyanopropyl stationary phase

• Compatible with conventional HPLC systems

• Industry-standard 4.6 mm ID analytical format

• Longer column length provides greater resolving power

• Ideal replacement for many legacy cyano methods

• Alternative selectivity to traditional C18 columns

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