Catalog Number - 64418-15P-2

Nova Similars C18™ is a traditional reversed-phase HPLC stationary phase manufactured on high-purity Type-B spherical silica with a fully endcapped octadecyl (C18) bonded phase. Designed as a cost-effective alternative to Waters™ Nova-Pak® columns, it offers comparable chromatographic behavior for USP L1 methods while providing the strong hydrophobic retention, reproducibility, and broad applicability expected from a conventional C18 stationary phase.

64418-15P-2 is a 150 mm x 2.1 mm ID narrow-bore analytical column packed with 4 µm Nova Similars C18 particles. The 150 mm length provides increased resolving power for more demanding separations, while the 4 µm particles deliver higher efficiency and somewhat sharper peaks than comparable 5 µm columns. The 2.1 mm ID format minimizes solvent consumption and is well suited for LC-MS applications. Typical operating flow rates are approximately 0.2–0.3 mL/min and require a system capable of precise low-flow delivery with low-volume flow cells and plumbing.

This USP L1 column is ideal for hydrophobic and neutral compounds requiring strong reversed-phase retention. Common applications include LC-MS methods, pharmaceutical analysis, long-chain fatty acid separations, and method development where increased sensitivity, efficiency, and chromatographic resolution are desired.

Key Benefits

• Type-B spherical silica stationary phase

• Suitable alternative for USP L1 methods

• Comparable selectivity to Waters™ Nova-Pak® C18 columns

• Narrow-bore format ideal for LC-MS applications

• Increased resolving power from 150 mm length

• Higher efficiency than comparable 5 µm columns

• Reduced solvent consumption

• End-capped C18 bonded phase

Typical Applications

• USP L1 methods

• LC-MS analysis

• Reversed-phase HPLC

• Pharmaceutical analysis

• Long-chain fatty acid analysis

• Method development

• Quality control testing

• Method transfer and validation

Specifications

• Product Type: HPLC Column

• Stationary Phase: Nova Similars C18

• USP Classification: L1

• Particle Size: 4 µm

• Particle Type: Type-B Spherical Silica

• Bonded Phase: C18 (Octadecyl)

• Endcapped: Yes

• Column Dimensions: 2.1 mm ID x 150 mm

• Length: 150 mm

• Recommended Flow Rate: 0.2–0.3 mL/min

• Brand: Cogent

Important Notes

• Designed for reversed-phase chromatography

• Narrow-bore format optimized for LC-MS applications

• Requires an HPLC or LC-MS system capable of reliable low-flow operation

• Higher efficiency than comparable 5 µm columns

• Reduced solvent consumption compared to 4.6 mm ID columns

• Excellent alternative for many USP L1 methods

• Increased resolving power for complex separations

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