HPLC Column, RP Phenyl, Wide Pore, 3um, 2.1mm ID x 150mm Length, 300A. Cogent Brand. 1 EA.
Additional Information:
Cogent RP Phenyl™ Wide Pore is a reversed-phase HPLC stationary phase designed to provide unique selectivity through π-π interactions between aromatic analytes and the bonded phenyl functionality while offering enhanced accessibility for larger molecules. Manufactured using a traditional three-carbon linker with a terminal phenyl ring bonded to ultra-high purity silica and fully double end capped, this 300 Å wide pore phase provides chromatographic behavior distinct from conventional alkyl phases. As a USP L11 equivalent phase, it is particularly useful for large molecules, aromatic compounds, natural products, peptides, and method development applications.
68324-15P-2-3M is an HPLC column packed with 3 µm RP Phenyl™ Wide Pore particles featuring a 300 Å pore size in a 2.1 mm ID x 150 mm format. This narrow-bore configuration combines maximum chromatographic resolution with reduced solvent consumption and excellent compatibility with LC-MS instrumentation. The 150 mm length provides significantly greater peak capacity and resolving power than shorter column formats, while the wide pore structure improves analyte access to the bonded phase for larger compounds. Typical operating flow rates are approximately 0.2–0.3 mL/min. Because of the low flow rates required by 2.1 mm ID columns, standard HPLC systems must be capable of precise and reproducible low-flow operation.
This column is particularly useful for the separation of large positional isomers, natural products, related compounds, and certain peptides in reversed-phase HPLC and LC-MS applications. The unique selectivity of the phenyl phase can provide improved resolution for aromatic analytes where conventional C8 or C18 phases may not deliver adequate selectivity. The combination of wide pores, high resolving power, and LC-MS compatibility makes this column an excellent choice for demanding analytical and bioanalytical methods.
Key Benefits
RP Phenyl™ Wide Pore stationary phase
USP L11 equivalent chemistry
Fully double end capped
Phenyl bonded phase
Ultra-high purity silica support
300 Å wide pore design
Enhanced aromatic selectivity
Optimized for larger molecules
3 µm particle size
Excellent for LC-MS applications
Typical Applications
USP L11 methods
LC-MS methods
Large positional isomer separations
Natural product analysis
Peptide analysis
Aromatic compound analysis
Method development
Bioanalytical methods
Pharmaceutical analysis
Reversed-phase HPLC
Specifications
Product Type: HPLC Column
Stationary Phase: RP Phenyl™ Wide Pore
USP Classification: L11
Chromatography Mode: Reversed Phase
Bonded Phase: Phenyl
Endcapping: Fully Double End Capped
Particle Size: 3 µm
Pore Size: 300 Å
Column Dimensions: 2.1 mm ID x 150 mm
Length: 150 mm
Typical Flow Rate: 0.2–0.3 mL/min
Brand: Cogent
Important Notes
Designed for larger molecules and aromatic compounds
300 Å wide pores improve access to the bonded phase for larger analytes
Suitable for conventional HPLC and LC-MS systems
The 2.1 mm ID format is commonly used for LC-MS applications
Standard HPLC systems must be capable of precise and reproducible operation at approximately 0.2–0.3 mL/min
Detector flow cells should be optimized for low-volume, narrow-bore chromatography
3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns
Generates higher backpressure than larger particle columns
The 150 mm length provides maximum resolution and peak capacity for complex separations
Provides unique selectivity compared to C8 and C18 phases
Particularly useful for peptides, natural products, and larger aromatic analytes