Catalog Number - 72530-05P-2

The 72530‑05P‑2 Cogent C30™ HPLC Column is designed for shape‑selective separation of long‑chain hydrophobic compounds, offering unique selectivity for positional and geometric isomers that cannot be resolved with traditional C18 phases.

This column features a 30‑carbon bonded stationary phase on high‑purity silica (non‑end capped), providing stronger hydrophobic interaction and an alternative selectivity mechanism under reversed‑phase conditions.

With a 200 Å pore size, it is well suited for larger molecules, including carotenoids, vitamins, peptides, and some proteins, while maintaining reliable chromatographic performance.

✅ Comparable selectivity and application performance to YMC® C30 columns, making it a dependable alternative for USP L62 methods and similar workflows

50 mm Length – Fast LC‑MS Screening & Method Development

The 50 mm length is optimized for rapid separations, making it ideal for screening and early method development.

• Short run times and fast gradients

• Efficient evaluation of selectivity and retention

• Lower solvent consumption

✅ Use when speed and method development flexibility are priorities

Flow Rate & System Requirements (2.1 mm ID, 5 µm)

⚠️ Requires low‑flow HPLC or LC‑MS system capability

• Typical flow rate range: ~0.2–0.3 mL/min

• Lower backpressure vs 3 µm versions

• Requires stable low‑flow pump control

• Compatible with low‑volume LC‑MS detectors

✅ Sample preparation requirement: filter samples using a 0.2 µm syringe filter prior to injection

Why Choose C30 for Hydrophobic Separations

Shape Selectivity for Isomers

• Resolves positional and geometric isomers

• Provides separation beyond C18 capability

Effective for Larger Molecules

• 200 Å pore improves interaction with complex analytes

• Ideal for carotenoids, vitamins, and peptides

Reversed‑Phase Alternative to Normal Phase

• Avoids instability and reproducibility issues

• Maintains robust, repeatable operation

Cogent C30™ Advantages

Extended C30 Ligand

• Stronger hydrophobic interaction vs C18

• Unique selectivity for long‑chain compounds

High Purity Silica

• Consistent performance and reproducibility

• Reliable chromatographic results

Method Transfer Compatibility

• Comparable to YMC® C30 column chemistry

• Suitable for L62 method transfer with minimal adjustment

Typical Applications

• Carotenoid and vitamin analysis

• Isomer and positional compound separations

• Peptide and large molecule separations

• Hydrophobic compound profiling

• LC‑MS method development

Important Usage Notes

• Designed for low‑flow LC‑MS systems (~0.2–0.3 mL/min)

• Not end‑capped (intentional for selectivity differences)

• Lower backpressure vs 3 µm columns

• Ideal for routine LC‑MS and method development workflows