Catalog Number - 40031-05P-2

40031-05P-2 Cogent UDA™ (weak cation exchange, WCX) HPLC Column is built on Cogent TYPE‑C™ silica (silica hydride platform), offering a highly unique and flexible separation mechanism not achievable with conventional bonded phases such as C18 or traditional ion exchange columns.

The UDA™ ligand (Undecanoic Acid) consists of an 11‑carbon hydrophobic chain with a terminal carboxylic acid group, enabling true mixed‑mode chromatography:

• Reversed phase (hydrophobic interactions)

• Weak cation exchange (WCX at pH ≥ 6)

• HILIC (polar retention mechanism)

This versatility allows powerful separations of basic, polar, ionizable, and structurally similar compounds, especially in complex matrices where traditional single‑mode columns fail.

The TYPE‑C silica hydride base provides important advantages:

• Direct silicon–carbon bonding for enhanced durability

• Ability to operate at very low pH (below 2) without damage

• Faster equilibration compared to conventional silica

• Reduced sensitivity to mobile phase moisture

50 mm Length – Fast LC‑MS and High‑Throughput Workflows

The 50 mm length is ideal for rapid analysis and method development.

• Very fast run times

• Reduced solvent consumption

• Ideal for screening and high‑throughput methods

✅ Recommended for LC‑MS workflows, rapid analysis, and method scouting

Flow Rate & System Requirements (2.1 mm ID, 4 µm)

⚠️ Requires low‑flow capable HPLC or UHPLC system

• Typical flow rate range: ~0.2 – 0.4 mL/min

• Moderate backpressure vs sub‑2 µm particles

• Requires accurate low‑flow delivery and low‑volume detection

✅ Important: Standard HPLC systems must be capable of stable low‑flow precision

✅ System Fit: Excellent for LC‑MS systems and low‑dispersion analytical setups

Unique Selectivity – Why Choose UDA™ (WCX)

True Multi‑Mode Retention

• Hydrophobic (RP)

• Ionic (weak cation exchange)

• Polar (HILIC)

Ideal for Complex Samples

• Resolves co‑eluting compounds

• Effective for basic and positively charged analytes

pH‑Controlled Selectivity

• WCX functionality active at pH ≥ 6

• Retention tunable via pH and ionic strength

✅ Ideal when C18 or standard ion exchange columns do not provide adequate separation

TYPE‑C Silica Advantages

Extreme Robustness

• Stable under very low pH conditions (<2)

• Resistant to hydrolysis vs traditional silica

Faster Equilibration

• Reduces run cycle time

No Strict Moisture Control in HILIC

• Easier method development

Extended Column Lifetimes

• Durable for demanding analytical applications

Cogent UDA™ Column Advantages

Mixed‑Mode Flexibility

• Combines RP, HILIC, and WCX in one column

Balanced Performance (4 µm Particles)

• Lower pressure than UHPLC columns

• Suitable for broader system compatibility

Optimized for LC‑MS

• Minimal salt required (HILIC mode)

• MS‑friendly separations

Typical Applications

• LC‑MS method development

• Complex matrix separations

• Basic and positively charged compounds

• Polar and ionizable analytes

• Pharmaceutical and bioanalytical workflows

Important Usage Notes

• Packed and shipped in reversed‑phase solvents

• Requires low‑flow capable HPLC or UHPLC system

• Ideal for LC‑MS and low‑dispersion systems

• pH control is critical for WCX functionality