Catalog Number - 40060-05P-2

The 40060‑05P‑2 Diol HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, providing excellent selectivity, faster equilibration, and robust column lifetime compared to conventional bonded‑silica diol phases. The 4 µm silica hydride particles offer reliable performance with moderate backpressure, making this column well suited for routine analytical workflows and LC‑MS applications.

Built on the TYPE‑C™ surface, this Diol column delivers stable retention behavior and reproducible performance across reversed‑phase, normal‑phase, and HILIC methods.

Application Focus: Diol Chemistry Across HILIC, NP & RP

Diol stationary phases are commonly selected for the analysis of polar compounds that are difficult to retain in reversed‑phase chromatography. This column is effective for a broad range of analytes, including:

• Polar and non‑polar peptides

• Amino acids

• Polar metabolites

• Small biological and biomolecular compounds

The balanced polarity of the Diol phase supports multiple chromatographic modes, allowing method flexibility without changing column chemistry.

Cogent TYPE‑C™ Silica Hydride Advantages

Compared to traditional bonded‑silica diol columns, the TYPE‑C™ silica hydride surface offers meaningful operational advantages:

• Faster equilibration, particularly in HILIC methods

• Excellent selectivity for polar and mixed‑polarity analytes

• Improved robustness and column lifetime

• Reduced susceptibility to silica hydrolysis

These characteristics make the 40060‑05P‑2 a dependable choice for routine use and method development.

Why Choose the 2.1 mm × 50 mm Format

The short, narrow‑bore geometry is optimized for speed, solvent efficiency, and LC‑MS compatibility:

• Short run times for high sample throughput

• Lower solvent consumption and waste

• Improved ionization efficiency with reduced flow to the MS source

• Compact column volume, supporting fast equilibration

This size is a very popular choice for LC‑MS, particularly for screening and targeted analyses of polar compounds.

Particle Size and Performance

The 4 µm particle size provides a practical balance of efficiency, robustness, and backpressure. While lower in efficiency than 2.2 µm formats, it offers:

• Reliable separation performance

• Broader system compatibility

• Lower pressure requirements

This makes the column suitable for laboratories prioritizing durability and ease of use alongside analytical performance.

LC‑MS‑Focused Operation

The 2.1 mm internal diameter supports LC‑MS workflows by:

• Delivering sharp, well‑defined peaks

• Reducing solvent load to the MS source

• Supporting consistent quantitative results

The column’s versatility across RP, NP, and HILIC modes further enhances its value for LC‑MS laboratories.

System Requirements and Flow Rates

• Typical flow range (2.1 mm ID): ~0.2–0.3 mL/min

• System capability: Requires an HPLC system capable of stable, accurate low‑flow delivery

• Important note: Not all standard HPLC systems are optimized for precise low‑flow operation

Flow‑rate optimization should consider solvent composition, viscosity, column temperature, and system pressure limits.