Catalog Number - 40200-05P-2

The 40200‑05P‑2 Silica‑C™ 2.o HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, delivering a “near‑UHPLC” level of performance with 2.2 µm particles and 120 Å pore size under high‑pressure analytical conditions. Unlike conventional bonded‑silica columns, the silica hydride surface provides faster equilibration, improved robustness, and greater method stability, particularly in HILIC and normal‑phase separations.

This column is optimized for applications where non‑aqueous or low‑water mobile phases are required and where traditional silica phases present equilibration or moisture‑control challenges.

Application Focus: Silica‑C™ for HILIC & Normal‑Phase Separations

Silica‑C™ stationary phases are selected for the analysis of compounds that are poorly soluble in water or require non‑polar or minimally aqueous mobile phases. The Silica‑C™ 2.o phase is particularly effective for:

• Lipids

• Peptides and proteins

• Oligonucleotides

• Other compounds requiring non‑polar solvents or HILIC conditions

The TYPE‑C™ silica hydride surface provides enhanced stability and reproducibility compared to traditional silica, supporting reliable retention without the variability often caused by water adsorption.

Key Benefits of Silica‑C™ TYPE‑C™ Technology

Compared to conventional silica columns, Silica‑C™ columns provide:

• Faster equilibration between runs

• No need to control moisture in mobile‑phase solvents

• Robust column lifetime under demanding solvent systems

• Improved method reproducibility in HILIC and normal‑phase workflows

These advantages make Silica‑C™ a preferred choice for laboratories seeking consistent performance in non‑aqueous separations.

Why Choose the 2.1 mm × 50 mm Format

The 2.1 mm ID × 50 mm length configuration is optimized for speed, efficiency, and LC‑MS compatibility:

• Short run times and high sample throughput

• Rapid equilibration between injections

• Reduced solvent consumption

• Lower flow to the detector or MS source, improving sensitivity

This narrow‑bore format is especially attractive for LC‑MS workflows where solvent efficiency and fast analysis are critical.

Particle Size and Efficiency

The 2.2 µm particle size delivers high separation efficiency and sharp peak shapes under high‑pressure operation, providing near‑UHPLC performance while remaining compatible with many modern analytical HPLC systems.

System Requirements and Flow Rates

• Typical flow range (2.1 mm ID): ~0.2–0.3 mL/min

• System capability: Requires an HPLC system capable of accurate, stable low‑flow delivery

• Important note: Not all standard HPLC systems are suitable for reliable operation at these reduced flow rates

Final flow‑rate selection should consider mobile‑phase viscosity, column temperature, and system pressure limits.