Catalog Number - 69220-15P-3

The 69220‑15P‑3 Phenyl Hydride 2.o HPLC Column is manufactured using Cogent TYPE‑C™ silica hydride technology, combining a phenyl‑selective surface with a silica hydride backbone to provide excellent selectivity, fast equilibration, and robust column lifetime under high‑pressure analytical conditions. The 2.2 µm particle size delivers near‑UHPLC separation efficiency while remaining compatible with standard analytical HPLC systems.

This column is produced as a custom, made‑to‑order item and is optimized for applications requiring enhanced aromatic‑specific selectivity beyond conventional reversed‑phase materials.

Application Focus: Phenyl Hydride Selectivity for Aromatic Compounds

Phenyl Hydride chemistry is selected for analytes containing aromatic rings that are difficult to retain or resolve using traditional reversed‑phase columns. The Phenyl Hydride 2.o phase enhances selectivity through π–π interactions in addition to hydrophobic retention, delivering improved resolution for challenging aromatic compounds.

This column is particularly effective for:

• Aromatic amines

• Small peptides with aromatic residues

• Azides and substituted aromatic compounds

• Other analytes benefiting from phenyl‑based selectivity

The TYPE‑C™ silica hydride surface enables faster equilibration and greater stability compared to conventional bonded‑silica phenyl phases.

Why Choose the 3.0 mm × 150 mm Format

The 3.0 mm ID × 150 mm length configuration offers a balance of high resolution and analytical efficiency, delivering performance comparable to traditional 4.6 mm ID columns while reducing operating costs and solvent usage:

• Comparable run times to 4.6 mm ID analytical columns

• Lower mobile‑phase consumption

• Reduced operating flow rates

• Lower backpressure, despite small particle size

This format provides enhanced resolution without requiring specialized low‑flow or UHPLC‑only instrumentation.

Particle Size and Efficiency

The 2.2 µm particle size delivers high efficiency and narrow peak widths under high‑pressure operation, improving resolution of closely eluting aromatic compounds while maintaining flexibility on standard analytical HPLC platforms.

LC‑MS and Analytical Performance

Reduced solvent usage and stable Phenyl Hydride retention behavior make this column well suited for LC‑MS and LC‑MS/MS workflows involving aromatic compounds, supporting:

• Symmetrical peak shapes

• Improved sensitivity

• Reliable quantitative repeatability across extended gradients

System Requirements and Flow Rates

• Typical flow range (3.0 mm ID): ~0.4–0.6 mL/min

• System capability: Compatible with standard analytical HPLC systems

• Note: No special low‑flow or UHPLC‑only equipment is required

Final flow rates should be optimized based on mobile‑phase viscosity, column temperature, and system pressure limits.

Made‑to‑Order Columns & Pricing Information

This is a custom, made‑to‑order column.

• Columns are manufactured specifically to order

• Orders cannot be canceled once placed

• Custom columns are non‑returnable

Please contact Customer Service for formal quotation, lead time, and Please contact Customer Service using our online form or by phone to initiate the quotation process.

• View more information on Custom Items.