High‑Purity Silica Columns for Normal‑Phase and HILIC Methods
USP® L‑03 | Excellent for Fat‑Soluble Analytes | Wide Particle Size Range
Cogent NP™ Silica HPLC columns are designed for normal‑phase (NP) and HILIC chromatography, providing reliable, reproducible performance for fat‑soluble analytes and polar compounds. These columns are manufactured using the same high‑purity silica and narrow particle size distribution used in Cogent RP™ columns, ensuring consistent quality and efficiency.
As USP® L‑03 classified silica columns, Cogent NP™ Silica is a dependable choice for both compendial methods and routine laboratory workflows.
High‑Purity, Unmodified Silica for NP and HILIC
Cogent NP™ Silica columns are unmodified, with no carbon loading, preserving the traditional silica surface required for normal‑phase separations. The silica features metal content below 1 ppm, which helps reduce unwanted interactions and contributes to excellent column efficiency and durability.
This controlled silica chemistry delivers:
Strong polar retention
Predictable selectivity
High efficiency
Excellent reproducibility
Designed for Normal‑Phase and HILIC Chromatography
Cogent NP™ Silica columns provide excellent performance in both NP and HILIC modes, offering flexibility for laboratories working across different separation strategies.
They are especially well suited for:
Normal‑phase methods using non‑polar mobile phases
HILIC methods using acetonitrile and water
Applications where reversed‑phase retention is insufficient
This versatility allows analysts to adapt a single silica chemistry to multiple workflows.
Ideal for Fat‑Soluble and Polar Compounds
Cogent NP™ Silica columns are commonly selected for the separation of:
Fats and lipids
Fat‑soluble vitamins
Steroidal compounds
Polar organic molecules
Analytes specified in USP® L‑03 methods
The unmodified silica surface provides the strong polar interactions required for these challenging separations.
Broad Range of Particle Sizes and Formats
To support both method development and scale‑up, Cogent NP™ Silica columns are available in a wide range of particle sizes and column dimensions, including options with wide average pore size (300 Å) for larger molecules.
This flexibility allows laboratories to:
Optimize efficiency and resolution
Transfer methods confidently
Scale from analytical to preparative HPLC
Key Features and Benefits
Designed for USP® L‑03 silica methods
Optimized for normal‑phase and HILIC chromatography
Unmodified, high‑purity silica
Metal content below 1 ppm
Narrow particle size distribution
Excellent efficiency and reproducibility
Available in a wide range of particle sizes
Optional 300 Å porosity for larger analytes
Suitable for analytical and preparative HPLC
Typical Applications
Normal‑phase HPLC separations
HILIC methods
Fat‑soluble compounds and vitamins
Steroids and lipid‑like molecules
Polar analyte separations
USP® L‑03 compliant methods
Additional Resources
Column Protection Recommendation
Important: MICROSOLV technical support strongly recommends using the Cogent Column Filter, which is included with each column, whenever possible. Column filters help protect against particulates, extend column lifetime, and maintain consistent chromatographic performance.
👉 Learn more about the Cogent Column Filter for HPLC column protection
